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Astrocyte Medium (AM) consists of 500 ml of basal medium, 10 ml of fetal bovine serum (FBS, Cat. No. 0010), 5 ml of astrocyte growth supplement (AGS, Cat. No. 1852) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).

PM consists of 500 ml of basal medium, 10 ml of FBS (Cat. #0010), 5 ml of Pericyte Growth Supplement (PGS, Cat. #1252) and 5 ml of penicillin/streptomycin solution (P/S, Cat. #0503). Note: FBS, PGS and P/S are not pre-mixed in PM; they must be added separately to make the complete PM.

Epithelial Cell Medium (EpiCM) consists of 500 ml of basal medium, 10 ml of FBS (Cat. #0010), 5 ml of Epithelial Cell Growth Supplement (EpiCGS, Cat. #4152) and 5 ml of penicillin/streptomycin solution (P/S, Cat. #0503).

Endothelial Cell Medium (ECM) consists of 500 ml of basal medium, 25 ml of FBS (Cat. #0025), 5 ml of Endothelial Cell Growth Supplement (ECGS, Cat. #1052) and 5 ml of penicillin/streptomycin solution (P/S, Cat. #0503). Note: FBS, ECGS and P/S are not pre-mixed in ECM; they must be added separately to make the complete ECM.

Trypsin Neutralization Solution (TNS) is used to neutralize the effects of Trypsin/EDTA solution (Cat. No. 0103) after the release of cells from a culture surface. It is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Fibroblast Medium (FM) consists of 500 ml of basal medium, 10 ml of fetal bovine serum (FBS, Cat. No. 0010), 5 ml of fibroblast growth supplement (FGS, Cat. No. 2352), and 5 ml of Antibiotic solution (P/S, Cat. No. 0503).

Isolated from human brain. HBMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume.

0.25% Trypsin/EDTA is used to detach adherent cells from a culture surface. T/E is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

0.05% Trypsin/EDTA is used to detach adherent cells from a culture surface. T/E is for research use only.  It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

DMEM/F-12 with L-Glutamine and HEPES (DMEM/F12) is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.