GeneQuery™ HLA-A PCR+Sanger SBT Typing Kit
Catalog No.
GK891
GeneQuery™ HLA-A PCR+Sanger SBT Typing Kit (SBT-A) utilizes the Sequence-Based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.
Kit Components:
| Cat # | Component | Quantity | Storage |
| GK891a | HLA-A amplification primer set (A_ampF and A_ampR) | 400 µL | -20°C |
| GK891b | A_2F sequencing primer mix, 10 µM | 200 μL | -20°C |
| GK891c | A_2R sequencing primer mix, 10 µM | 200 μL | -20°C |
| GK891d | A_3F sequencing primer mix, 10 µM | 200 μL | -20°C |
| GK891e | A_3R sequencing primer mix, 10 µM | 200 μL | -20°C |
| GK891f | A_4F sequencing primer mix, 10 µM | 200 µL | -20°C |
| GK891g | A_4R sequencing primer mix, 10 µM | 200 μL | -20°C |
| GK891h | Nuclease-free H2O | 4 mL | 4°C |
| GK891r | Human genomic DNA reference (GDR), ~50 ng/µL (HLA-A*02:01 and HLA-A*25:01) |
10 μL | -20°C |
| MB6038-1 | 2X EmeraldNStart HiFi Marathon PCR Master Mix, 1 mL | 2 vials | -20°C |
$682.00
In Stock
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