Explore Immune Cell Dynamics with GeneQuery™ qPCR Array Kits! 

Microglial & Macrophage Polarization Marker Profiling – Simplified and Streamlined

Understanding how immune cells respond to their environment is critical for uncovering the mechanisms behind inflammation, neurodegeneration, cancer, and more. The GeneQuery™ 

Polarization Marker qPCR Array Kits offer a fast, reliable solution for targeted gene expression profiling of 40 key polarization markers.

These pre-validated qPCR arrays are ideal for researchers studying cell phenotype switching in response to stimuli, providing clear insights with minimal hands-on time and no need for gene selection or primer optimization.

GeneQuery™ Human Microglial Polarization Markers Kit (Cat. #GK114)

Microglia, the brain’s resident immune cells, can adopt distinct phenotypes based on their activation state. This kit enables profiling of the transcriptional signatures associated with:

• M1 Microglia (Pro-inflammatory) – Defend against pathogens through cytokine production and inflammation.
  Includes markers like TNF, IL1B, IL6, NOS2, FCGR2A/B/C, FCGR3A/B, CD86.
• M2 Microglia (Anti-inflammatory) – Promote tissue repair, resolve inflammation, and support CNS homeostasis.

Includes markers such as MRC1, IL4, IL10, TGFB1, IL1R1, SOCS3, ARG1, IL13, SPHK1.

This kit is ideal for studies on neuroinflammation, neurodegenerative disorders, and CNS injury models.

GeneQuery™ Human Macrophage Polarization Markers Kit (Cat. #GK120)

Macrophages are highly plastic immune cells that shift their function depending on environmental cues. This kit covers:

• M1 (Classically Activated) – Pro-inflammatory and pathogen-fighting functions.
• M2 (Alternatively Activated) – Tissue repair and immune regulation.

Includes M2 subsets: M2a, M2b, M2c, and M2d with diverse immunomodulatory roles.

Tumor-Associated Macrophages (TAMs) – Often exhibit immunosuppressive traits and support tumor growth.

Markers include well-characterized genes such as CD163, IL10, IL1R1, TGFB1, ARG1, CXCL10, SOCS3, and others.

Use this kit to support research in chronic inflammation, fibrosis, cancer immunology, and tissue remodeling.

Key Features of GeneQuery™ qPCR Array Kits

The GQH-MPM (Microglial) and GQH-MPP (Macrophage) kits are designed for efficient, high-confidence gene expression analysis:

• qPCR-ready 96-well format with pre-validated primer sets targeting specific genes
• Optimized primers with a 65°C annealing temperature, covering all known transcript variants (unless noted), and specific to a single gene
• Eight built-in controls, including: 
  • 5 housekeeping genes (ACTB, GAPDH, LDHA, NONO, PPIH) for accurate normalization
  • Genomic DNA Control (GDC) for contamination detection
  • Positive PCR Control (PPC) and No Template Control (NTC) for PCR quality assurance
• User-friendly protocol with a standard 3-step qPCR setup and detailed analysis guidance
• Supports ∆∆Cq data analysis using all five housekeeping genes (geometric mean recommended)
• Compatible with major qPCR systems (ABI/Life Technologies, Bio-Rad, Eppendorf, Stratagene, Roche), with plate types A, B, and C available

   

Ready to accelerate your immune profiling studies?
Visit sciencellonline.com or contact us for more details, bulk pricing, or custom array options.

One kit = everything you need to test 40 genes in two samples.