3D-Osteoblast Spheroid Medium

3D-OSpM consists of 200 ml of basal medium, 10 mL of FBS (Cat. #0010), 2 mL of 3D-osteoblast spheroid supplement (3D-OSpS, Cat. #3D-4652), and 2 mL penicillin/streptomycin solution (P/S, Cat. #0583). Note: 3D-OSpS, FBS, and P/S are not pre-mixed in the 3D-OSpM; they must be added separately to make the complete medium.