3D-Neuronal Spheroid Medium

3D-NSpM consists of 200 mL of basal medium, 4 mL of 3D-neuronal spheroid supplement (3DNSpS, Cat. #3D-1562), and 2 mL penicillin/streptomycin solution (P/S, Cat. #0583). Note: 3DNSpS and P/S are not pre-mixed in the 3D-NSpM; they must be added separately to make the complete medium.