来源于 C57BL/6 小鼠的肺成纤维细胞

The most abundant cell type in the lung interstitium is the fibroblast. Pulmonary fibroblasts (PF) exhibit typical fibroblast morphology, however, they also possess some distinguishing features such as long, branching processes and gap junctions. PF function to produce type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. PF also play an important role in the repair and remodeling processes following tissue damage. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury. An inadequate or an excessive accumulation of fibroblasts results in abnormal tissue function and can lead to the development of pulmonary fibrosis. Notably, pulmonary fibrosis has been observed in acute COVID-19 patients, however, little is known about how the fibrosis will affect lung function in recovered patients. The culture of mouse PF (MPF) in vitro can be used to further elucidate the mechanisms of pulmonary fibrosis and other types of lung disorders.

MPF from ScienCell Research Laboratories are isolated from C57BL/6 mouse lung. MPF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. MPF are characterized by immunofluorescence with antibody specific to fibronectin. MPF are negative for mycoplasma, bacteria, yeast, and fungi. MPF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing MPF in vitro.

肺间质中含量最丰富的细胞类型是成纤维细胞。肺成纤维细胞（PF）具有典型的成纤维细胞形态，同时还具有一些特征性结构，例如长而分支的突起和缝隙连接。PF 可产生肺泡隔细胞外基质中的 III 型胶原蛋白、弹性蛋白和蛋白聚糖。PF 还在组织损伤后的修复和重塑过程中发挥重要作用。成纤维细胞向炎症部位的有序募集对于损伤后的有效组织修复至关重要。成纤维细胞募集不足或过度聚集均可导致组织功能异常，并可能促进肺纤维化的发生。值得注意的是，急性 COVID-19 患者中已观察到肺纤维化现象，但目前对于纤维化将如何影响康复后患者的肺功能仍知之甚少。小鼠肺成纤维细胞（MPF）的体外培养可用于进一步阐明肺纤维化及其他肺部疾病的发生机制。

ScienCell Research Laboratories 提供的小鼠肺成纤维细胞（MPF）分离自 C57BL/6 小鼠肺组织。MPF 在第 1 代（P1）进行冻存，并以冷冻状态提供。每瓶含有 >5 × 10⁵ 个细胞，体积为 1 ml。MPF 采用针对纤连蛋白（fibronectin）的特异性抗体进行免疫荧光表征。MPF 的支原体、细菌、酵母及真菌检测结果均为阴性。根据 ScienCell Research Laboratories 提供的培养条件，MPF 可保证继续扩增 5 个群体倍增。

推荐培养基

推荐使用成纤维细胞培养基（Fibroblast Medium，FM，产品编号：2301）进行 MPF 的体外培养。

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