来源于 CD1 小鼠的淋巴成纤维细胞

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in various organs are intrinsically different. In the lymphoid compartment, fibroblasts construct a unique conduit system with intriguing size exclusion which plays a fundamental role in regulating immune response and homeostasis of tissue fluids.

MLF from ScienCell Research Laboratories are isolated from C57BL/6 mouse lymph node. MLF are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. MLF are characterized by their spindle-shaped morphology and immunofluorescence with antibody specific to fibronectin. MLF are negative for mycoplasma, bacteria, yeast, and fungi. MLF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing MLF in vitro.

成纤维细胞是来源于胚胎中胚层的间充质细胞。由于成纤维细胞较易在体外培养，因此被广泛应用于多种细胞和分子生物学研究。其较强的耐受性也使其适用于多种实验操作，包括基因转染和显微注射等研究。一般而言，成纤维细胞可分泌非刚性的细胞外基质，其中富含 I 型和/或 III 型胶原蛋白。研究表明，不同器官中的成纤维细胞在生物学特性上存在内在差异。在淋巴组织中，成纤维细胞可构建具有独特尺寸选择性的管道系统，在调节免疫反应和组织液稳态中发挥重要作用。

ScienCell Research Laboratories 提供的小鼠淋巴成纤维细胞（MLF）分离自 C57BL/6 小鼠淋巴结。MLF 在 P0 代进行冻存，并以冷冻状态提供。每瓶含有 >5 × 10⁵ 个细胞，体积为 1 ml。MLF 根据其梭形细胞形态，并采用针对纤连蛋白（fibronectin）的特异性抗体进行免疫荧光表征。MLF 的支原体、细菌、酵母及真菌检测结果均为阴性。根据 ScienCell Research Laboratories 提供的培养条件，MLF 可保证继续扩增 5 个群体倍增。

推荐培养基

推荐使用成纤维细胞培养基（Fibroblast Medium，FM，产品编号：2301）进行 MLF 的体外培养。

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