GeneQuery™ HLA-C PCR+Sanger SBT Typing Kit

Name: GeneQuery™ HLA-C PCR+Sanger SBT Typing Kit
Brand: Sciencell Research Laboratories
SKU: GK893
Price: 682 USD
Availability: InStock

Catalog No.

GK893

率先评论此产品

GeneQuery™ HLA-C PCR+Sanger SBT Typing Kit (SBT-C) utilizes the Sequence-based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.

Kit Components:

Cat #	Component	Quantity	Storage
GK893a	HLA-C amplification primer set (C_ampF and C_ampR)	400 µL	-20°C
GK893b	C_2F sequencing primer mix, 10 µM	200 μL	-20°C
GK893c	C_2R sequencing primer mix, 10 µM	200 μL	-20°C
GK893d	C_3F sequencing primer mix, 10 µM	200 μL	-20°C
GK893e	C_3R sequencing primer mix, 10 µM	200 μL	-20°C
GK893f	C_4F sequencing primer mix, 10 µM	200 µL	-20°C
GK893g	C_4R sequencing primer mix, 10 µM	200 μL	-20°C
GK893h	Nuclease-free H₂O	4 mL	4°C
GK893r	Human genomic DNA reference (GDR), ~50 ng/µL (HLA-C07:01 and HLA-C12:03)	10 μL	-20°C
MB6038-1	2X EmeraldNStart HiFi Marathon PCR Master Mix, 1 mL	2 vials	-20°C