GeneQuery™ HLA-B PCR+Sanger SBT Typing Kit

Name: GeneQuery™ HLA-B PCR+Sanger SBT Typing Kit
Brand: Sciencell Research Laboratories
SKU: GK892
Price: 682 USD
Availability: InStock

Catalog No.

GK892

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GeneQuery™ HLA-B PCR+Sanger SBT Typing Kit (SBT-B) utilizes the Sequence-Based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.

Kit Components:

Cat #	Component	Quantity	Storage
GK892a	HLA-B amplification primer set (B_ampF and B_ampR)	400 µL	-20°C
GK892b	B_2F sequencing primer mix, 10 µM	200 μL	-20°C
GK892c	B_2R sequencing primer mix, 10 µM	200 μL	-20°C
GK892d	B_3F sequencing primer mix, 10 µM	200 μL	-20°C
GK892e	B_3R sequencing primer mix, 10 µM	200 μL	-20°C
GK892f	B_4F sequencing primer mix, 10 µM	200 µL	-20°C
GK892g	B_4R sequencing primer mix, 10 µM	200 μL	-20°C
GK892h	Nuclease-free H₂O	4 mL	4°C
GK892r	Human genomic DNA reference (GDR), ~50 ng/µL (HLA-B08:01 and HLA-B18:01)	10 μL	-20°C
MB6038-1	2X EmeraldNStart HiFi Marathon PCR Master Mix, 1 mL	2 vials	-20°C