3D-Hepatic Stellate Cell Spheroid Medium

3D-SteCSpM consists of 200 ml of basal medium, 4 mL of FBS (Cat. #0004), 2 mL of 3D-stellate cell spheroid supplement (3D-SteCSpS, Cat. #3D-5352) and 2 mL penicillin/streptomycin solution (P/S, Cat. #0583). Note: 3D-SteCSpS, FBS and P/S are not pre-mixed in the 3D-SteCSpM; they must be added separately to make the complete medium.