3D-Airway Spheroid Medium

3D-ASpM consists of 200 ml of basal medium, 2 mL of 3D-airway spheroid supplement (3DASpS, Cat. #3D-3262), and 2 mL penicillin/streptomycin solution (P/S, Cat.#0583). Note: 3DASpS and P/S are not pre-mixed in the 3D-ASpM; they must be added separately to make the complete medium.