Serum in Cell Culture Today
Scientists are trying to reduce or replace animal serum in culture media due to ethical concerns, rising costs, batch variability, and concerns over infectious agents…
Telomeres aging and Telomere Length Quantification by qPCR.
Aging and Telomere Length Quantification by qPCR Aging is a time-dependent decline of the body’s functional capabilities and is an inevitable course of life (as shown in the image… Read more »
Not All Primary Cells are Created Equal.
Primary cells, which are isolated directly from tissue, show normal cell morphology and maintain many of the important markers and functions seen in vivo. Primary cells, though, have a finite lifespan and limited expansion capacity, so it is critical to use primary cells at low passage for your research.
Primary Neurons vs Neuronal Cell Lines
Neuronal cell lines are commonly used for in vitro neurobiology studies because they are more easily transfected compared to primary neurons and they proliferate, whereas primary neurons do not. Neuronal… Read more »
Circadian Clocks and its connection to Breast Cancer
The 2017 Nobel Prize in Physiology or Medicine was awarded to Jeffrey C Hall, Michael Rosbash, and Michael W Young for research that established key mechanistic principles on how circadian… Read more »
Value of qPCR in the Next Generation Sequencing Era
qPCR is still considered the gold standard for gene expression analysis, especially when template copy number is low. In short, NGS provides a great tool for analyzing a large number of genes to narrow down the research interest and qPCR provides a fast, reliable, and cost effective means to verify NGS results.