GeneQuery™ HLA-DPB1 PCR+Sanger SBT Typing Kit

Name: GeneQuery™ HLA-DPB1 PCR+Sanger SBT Typing Kit
Brand: Sciencell Research Laboratories
SKU: GK894
Price: 682 USD
Availability: InStock

Catalog No.

GK894

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GeneQuery™ HLA-DPB1 PCR+Sanger SBT Typing Kit (SBT-DPB1) utilizes the Sequence-based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.

Kit Components:

Cat #	Component	Quantity	Storage
GK894a	HLA-DPB1 amplification primer set (P_ampF and P_ampR)	400 µL	-20°C
GK894b	P_2F sequencing primer mix, 10 µM	200 μL	-20°C
GK894c	P_2R sequencing primer mix, 10 µM	200 μL	-20°C
GK894d	P_3F sequencing primer mix, 10 µM	200 μL	-20°C
GK894e	P_3R sequencing primer mix, 10 µM	200 μL	-20°C
GK894h	Nuclease-free H₂O	4 mL	4°C
GK894r	Human genomic DNA reference (GDR), ~50 ng/µL (HLA-DPB101:01 and HLA-DPB102:01)	10 μL	-20°C
MB6038-1	2X EmeraldNStart HiFi Marathon PCR Master Mix, 1 mL	2 vials	-20°C