GeneQuery™ HLA-A PCR+Sanger SBT Typing Kit

Name: GeneQuery™ HLA-A PCR+Sanger SBT Typing Kit
Brand: Sciencell Research Laboratories
SKU: GK891
Price: 682 USD
Availability: InStock

Catalog No.

GK891

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GeneQuery™ HLA-A PCR+Sanger SBT Typing Kit (SBT-A) utilizes the Sequence-Based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.

Kit Components:

Cat #	Component	Quantity	Storage
GK891a	HLA-A amplification primer set (A_ampF and A_ampR)	400 µL	-20°C
GK891b	A_2F sequencing primer mix, 10 µM	200 μL	-20°C
GK891c	A_2R sequencing primer mix, 10 µM	200 μL	-20°C
GK891d	A_3F sequencing primer mix, 10 µM	200 μL	-20°C
GK891e	A_3R sequencing primer mix, 10 µM	200 μL	-20°C
GK891f	A_4F sequencing primer mix, 10 µM	200 µL	-20°C
GK891g	A_4R sequencing primer mix, 10 µM	200 μL	-20°C
GK891h	Nuclease-free H₂O	4 mL	4°C
GK891r	Human genomic DNA reference (GDR), ~50 ng/µL (HLA-A02:01 and HLA-A25:01)	10 μL	-20°C
MB6038-1	2X EmeraldNStart HiFi Marathon PCR Master Mix, 1 mL	2 vials	-20°C