3D-Hepatic Stellate-Endothelial Cell Spheroid Medium

3D-SECSpM consists of 200 ml of basal medium, 4 mL of 3D-sinusoidal endothelial cell spheroid supplement (3D-SECSpS, Cat. #3D-5452) and 2 mL penicillin/streptomycin solution (P/S, Cat. #0583). Note: 3D-SECSpS and P/S are not pre-mixed in the 3D-SECSpM; they must be added separately to make the complete medium.