3D-Sertoli Spheroid Medium

3D-SerSpM consists of 200 mL of basal medium, 10 mL of FBS (Cat. #0010), 2 mL of 3Dsertoli spheroid supplement (3D-SerSpS, Cat. #3D-4572), and 2 mL penicillin/streptomycin solution (P/S, Cat. #0583). Note: 3D-SerSpS, FBS, and P/S are not pre-mixed in the 3D-SerSpM; they must be added separately to make the complete medium.