ECM consists of 500 ml of basal medium, 25 ml of FBS (Cat. #0025), 5 ml of Endothelial Cell Growth Supplement (ECGS, Cat. #1052) and 5 ml of penicillin/streptomycin solution (P/S, Cat. #0503). Note: FBS, ECGS and P/S are not pre-mixed in ECM; they must be added separately to make the complete ECM.
ECM-r consists of 500 ml of basal medium, 25 ml of CS (Cat. #0025-cs), 5 ml of Endothelial Cell Growth Supplement-rat (ECGS-r, Cat. #1062) and 5 ml of penicillin/streptomycin solution (P/S, Cat. #0503).
3D-SECSpM consists of 200 ml of basal medium, 4 mL of 3D-sinusoidal endothelial cell spheroid supplement (3D-SECSpS, Cat. #3D-5452) and 2 mL penicillin/streptomycin solution (P/S, Cat. #0583). Note: 3D-SECSpS and P/S are not pre-mixed in the 3D-SECSpM; they must be added separately to make the complete medium.
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