ECM consists of 500 ml of basal medium, 25 ml of FBS (Cat. #0025), 5 ml of Endothelial Cell Growth Supplement (ECGS, Cat. #1052) and 5 ml of penicillin/streptomycin solution (P/S, Cat. #0503). Note: FBS, ECGS and P/S are not pre-mixed in ECM; they must be added separately to make the complete ECM.
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