{"id":164,"date":"2016-06-07T16:31:50","date_gmt":"2016-06-07T16:31:50","guid":{"rendered":"https:\/\/sciencellonline.com\/blog\/?p=164"},"modified":"2024-10-28T10:43:08","modified_gmt":"2024-10-28T17:43:08","slug":"the-transcriptome-age-or-a-young-scientists-illustrated-primer","status":"publish","type":"post","link":"https:\/\/sciencellonline.com\/blog\/the-transcriptome-age-or-a-young-scientists-illustrated-primer\/","title":{"rendered":"The Transcriptome Age: Or, A Young Scientist\u2019s Illustrated Primer"},"content":{"rendered":"<p>Despite being essential for any experiment involving DNA replication, people rarely give primers a second thought.\u00a0 To coincide with the launch of our new <a href=\"\/products-services\/genetics-genomics\/custom-services\/qpcr-primers-for-individual-genes\/\" target=\"_blank\" rel=\"noopener\">qPCR gene reference tool<\/a>, we\u2019ll be giving them a little more of the praise they deserve.<\/p>\n<div id=\"attachment_169\" style=\"width: 148px\" class=\"wp-caption alignleft\"><a href=\"https:\/\/sciencellonline.com\/blog\/wp-content\/uploads\/2016\/06\/Dr.-Kary-Mullis.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-169\" class=\" wp-image-169\" src=\"https:\/\/sciencellonline.com\/blog\/wp-content\/uploads\/2016\/06\/Dr.-Kary-Mullis.jpg\" alt=\"Dr Kary Mullis received the 1993 Nobel Prize in Chemistry for his contributions toward developing the PCR. \" width=\"138\" height=\"239\" srcset=\"https:\/\/sciencellonline.com\/blog\/wp-content\/uploads\/2016\/06\/Dr.-Kary-Mullis.jpg 241w, https:\/\/sciencellonline.com\/blog\/wp-content\/uploads\/2016\/06\/Dr.-Kary-Mullis-173x300.jpg 173w\" sizes=\"(max-width: 138px) 100vw, 138px\" \/><\/a><p id=\"caption-attachment-169\" class=\"wp-caption-text\">Dr Kary Mullis received the 1993 Nobel Prize in Chemistry for his contributions toward developing the PCR.<\/p><\/div>\n<p>Primers are the foundation for such grand purposes as diagnosing diseases, tracking epidemics, creating phylogenetic trees, genetic fingerprinting, and <a href=\"http:\/\/www.nature.com\/news\/mammoth-genomes-provide-recipe-for-creating-arctic-elephants-1.17462\" target=\"_blank\" rel=\"noopener\">cloning a woolly mammoth<\/a>.\u00a0 On a smaller but no-less grand scale, these simple 15-30 nucleotide sequences are widely used to assess cell phenotypes, identify signal transduction aberrancies, perform functional gene analyses, and study molecular pathologies.\u00a0 The most common research application of primers today is probably in performing the polymerase chain reaction (PCR) to amplify or replicate a targeted DNA sequence.\u00a0 Like the humble primer, the PCR\u2019s advent revolutionized scientific and medical research allowing such modern luxuries as non-invasive paternity tests, AIDS testing, and genealogy tracing.\u00a0 PCR is now so common that it is virtually indispensable, and one type of PCR, quantitative real-time PCR (qPCR), is now <a href=\"http:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0103917\" target=\"_blank\" rel=\"noopener\">considered to be the gold standard<\/a> for assaying gene expression.\u00a0 In addition to its general utility, qPCR has the widest dynamic range, is the most affordable, and <a href=\"http:\/\/splice-bio.com\/although-rna-seq-is-very-hip-right-now-is-it-really-worth-its-money\/\" target=\"_blank\" rel=\"noopener\">yields the least biased results<\/a> out of the current popular gene expression assays.\u00a0 Even if you start your gene expression analysis with a more elaborate assay like a microarray or RNA-seq, you will likely use qPCR to validate those results.<\/p>\n<p>ScienCell offers <a href=\"\/products-services\/genetics-genomics\/qpcr-array-kits\/\" target=\"_blank\" rel=\"noopener\">pre-designed qPCR array kits<\/a> as well as <a href=\"\/products-services\/genetics-genomics\/custom-services\/qpcr-primers-for-individual-genes\/\" target=\"_blank\" rel=\"noopener\">individual gene primers<\/a> for scientists performing gene expression analysis.\u00a0 If one of our existing array kits does not fit your needs, you have the option to alter it so that it does or build your own array using our new qPCR gene primers database that catalogs all of our validated primer sets.\u00a0 Our new gene database makes building your own array easy even if you\u2019re not particularly well-versed in certain fields because it organizes all of our gene primers by functions in cell biology, physiology, and pathology.\u00a0 Once you\u2019ve selected all the genes you want for your array, you can rearrange the layout of your genes to be exactly as you wish and then rest assured knowing that the primers you will receive from us are validated for optimal efficiency and efficacy in our labs.\u00a0 Even if you\u2019re not building a qPCR array, take a look at <a href=\"\/products-services\/genetics-genomics\/custom-services\/qpcr-primers-for-individual-genes\/\" target=\"_blank\" rel=\"noopener\">our database<\/a> because it doubles as a general reference tool for gene functions.<\/p>\n<p>&nbsp;<\/p>\n<p>Happy priming!<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Despite being essential for any experiment involving DNA replication, people rarely give primers a second thought.\u00a0 To coincide with the launch of our new qPCR gene reference tool, we\u2019ll be&#8230; <a href=\"https:\/\/sciencellonline.com\/blog\/the-transcriptome-age-or-a-young-scientists-illustrated-primer\/\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":7,"featured_media":196,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[47],"tags":[13,14,52,15,34,35,36,51,31,32,18,4,30],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - 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